Bacterial disease resistance in Arabidopsis through flagellin perception. Article ( PDF Available) in Nature () · May with. Bacterial disease resistance in Arabidopsis through flagellin perception. PUBLISHED: Apr CITE AS: Nature. Apr 15; () In Arabidopsis thaliana, the receptor kinase flagellin sensing 2 (FLS2) confers recognition of bacterial flagellin (flg22) and activates a manifold defense response. In plants that are resistant to these successful pathogens, plant PAMP perception system, whose components are often shared across.

Author: Mikajar Zuran
Country: Mozambique
Language: English (Spanish)
Genre: Medical
Published (Last): 20 September 2004
Pages: 162
PDF File Size: 16.13 Mb
ePub File Size: 9.91 Mb
ISBN: 383-1-29104-989-5
Downloads: 31072
Price: Free* [*Free Regsitration Required]
Uploader: Tauramar

Bacterial disease resistance in Arabidopsis through flagellin perception.

Even though simulations have shown that compound tests, which combine the properties of summary statistics, are not easily confounded by demographic effects Zeng et al. The change of affinity of the Stor genotype, located in LRR17, therefore suggests further portions of the ectodomain are involved in flg22 binding, either directly or via alteration of the protein conformation. This is somewhat surprising given that the parental alleles presented greater differences in flg22 binding.

Error bars in y direction represent three technical replicates of two independent pools of 12 plants. In Arabidopsis thalianathe receptor kinase flagellin sensing 2 FLS2 confers recognition of bacterial flagellin flg22 and activates a manifold defense response. Recommend FPrime to your bactfrial or information manager to request an extended free trial for all tgrough at your institution.

This association was also performed for each amino acid change but it failed to identify single changes explaining any of the two measures.

The FLS2 epitopes of N13 and MN47 most probably present lower affinity for the FLS2 antibody since they differ from the epitope harbored by Col-0, against which the antibody was developed. Natural allelic variation underlying a major fitness trade-off in Arabidopsis thaliana. Although fls2 mutant plants are as susceptible as the wild type when bacteria are infiltrated into leaves, they are more susceptible to the pathogen Pseudomonas syringae pv.

We considered population structure parameters when correlating quantitative flgbinding and flginduced inhibition of seedling growth. You hereby agree to indemnify and keep indemnified F, its affiliates, contractors and agents from and against any and all losses including without resistanec direct, indirect and consequential losscosts, claims, damages or expenses yhrough whatever nature and howsoever caused arising directly or indirectly from any breach of these Terms and Conditions or arising from the Material posted on this website or content contained in any email sent using the facilities provided by the website by you including without limitation as a result of any infringement of any intellectual property or other proprietary rights, libel, defamation, obscenity or the Material being otherwise unlawful.


You are a close professional associate of any of the authors e. Data represent three technical replicates of each of two biological replicates. RNA was extracted from lerception of 10—12 independent seedlings in two biological independent trials.

As a receptor kinase, FLS2 is composed of a Leucine-rich repeat LRR ectodomain, a single transmembrane domain, and a cytosolic kinase domain and is solely responsible for binding flg22 Chinchilla et al. Email alerts New issue alert. Incremental steps toward incompatibility revealed by Arabidopsis epistatic interactions modulating salicylic acid pathway activation.

This entry form currently does not support special characters.

The full sequence 1— bp includes bp of noncoding upstream region, two exons, one intron, and ends at the first predicted recombination breakpoint. Allele-specific assay reveals functional variation in the chalcone synthase promoter of Arabidopsis thaliana that is compatible with neutral evolution. Related articles in Web of Science Google Scholar.

Pyrosequencing quantification of relative allelic expression was performed on cDNA samples. By posting or uploading Material you warrant and represent that: Plants and animals recognize microbial invaders by detecting pathogen-associated molecular patterns PAMPs such as flagellin. Here we show that treatment of plants with flg22, a peptide representing the elicitor-active epitope of flagellin, induces the expression of numerous defence-related genes and triggers resistance to theough bacteria dlsease wild-type plants, but not in plants carrying mutations in the flagellin receptor gene FLS2.

The number of Pto DC bacteria colonizing the leaf was determined 4 days after inoculation of 28 genotypes for which flg22 binding was determined Atwell et al. Since fresh weight of individual genotypes presented a relatively low heritability, the QTLs at the FLS2 locus explain a nonnegligible part of the genetic variation segregating between parental strains.

Next, differential growth was calculated for each RIL line as described above for inhibition of seedlings growth, and the quantitative trait loci QTLs controlling its variation were mapped. At the molecular level, the action of natural diseqse for R protein diversification is sometimes apparent, but no typical evolutionary pattern can be defined for molecular diversity at R gene loci Michelmore and Meyers ; Bergelson et al.

Statistical-method for testing the neutral mutation hypothesis by DNA polymorphism. The experiment was conducted in at least three independent biological trials. There is indeed perceptiion diversity among pathogen strains in the effectors that they harbor Charity et al. Inhibition of seedling growth in either the presence of flg22 as a function of inhibition of seedling growth in the presence of elf Moreover, trade-offs between growth and development have gained attention.



This bp long region includes bp noncoding DNA upstream of the coding region, the signal peptide, and the N-terminal part of the LRR region. We therefore investigated how variation in flg22 binding may influence bacterial growth in planta. The flginduced inhibition of seedling growth depends not only on FLS2 but also on BAK1 and other unknown molecular components.

You are an Editor for the journal in which the article is published. Abstract Much is known about the evolution of plant immunity components rexistance against specific pathogen strains: Mutant fls2 and efr T-DNA insertion lines are in Col-0 genetic background filled circles and diamonds, respectively.

This suggests that one or several molecular variant s common to the two systems control some of the functional variation of PRRs. Although fls2 mutant plants are as susceptible as the wild type when bacteria are infiltrated into leaves, they are more susceptible to the pathogen Pseudomonas syringae pv. Specific binding to flg22 to Arabidopsis thalianaArabidopsis lyrataCardamine hirsuta, and Brassicaceae species. While homologues of FLS2 are present in diverse plant families, EFR is restricted to the Brassicaceae suggesting a more recent origin of this system Kunze et al.

Although the corresponding flg22 peptide flagelllin the Pto DC strain differs from the peptide used in the assay of flg22 binding by four amino acid residues in the N-terminal part, arabidoopsis still elicits a strong FLS2-mediated defense response Felix et al.

The phylogenetic analysis showed that alleles grouped in three major clades supported by elevated bootstrap values arabidolsis fig.

Bacterial disease resistance in Arabidopsis through flagellin perception.

FPrime is an expert-curated resource to help you find the articles of greatest interest and relevance to you. Pleiotropy modulates the efficacy of selection in Drosophila melanogaster. The action of variants at genes unknown to be related to flg22 perception but mapping close to the FLS2 locus, cannot be excluded. However, the importance of flagellin perception ressistance disease resistance has, until now, not been demonstrated.